Development of High-Concentration Collagen Bio-ink for High-Resolution 3D Bioprinting
Nippi Research Institute of Biomatrix has developed a
high-concentration Collagen Bio-ink that enables
high-resolution 3D bioprinting, overcoming the limitations of conventional collagen bio-ink. This innovative material is expected to significantly contribute to the fabrication of complex 3D tissue models and the development of new medical materials across a wide range of fields, including orthopedics, oral and maxillofacial surgery, and drug discovery research.
Background
In recent years, 3D bioprinting technology has attracted significant attention in the medical and drug discovery sectors as a powerful tool for reproducing the complex structures of biological tissues and organs. Collagen-based bio-inks are particularly advantageous as medical materials due to their
excellent biocompatibility and biodegradability. However, conventional collagen bio-inks present challenges in maintaining the printed shape and require precise temperature and pH control during the printing process.
Overview of the New Bio-ink
Nippi’s newly developed Collagen Bio-ink leverages proprietary technology to achieve
a collagen concentration exceeding 10%, significantly surpassing conventional products (typically 1–7%). This advancement is achieved while ensuring
stable extrusion through fine nozzles (inner diameter: 0.2 mm).
Crucially, this new bio-ink also offers
superior ease of use:
- Room Temperature Printing: Eliminates the need for complex temperature control (cooling or heating) required by conventional collagen bio-inks.
- Simplified Bioprinting: Enables easier and more efficient fabrication of high-resolution 3D collagen structures that were previously difficult to print.
- Cell Compatibility: The bio-ink’s neutral pH allows direct mixing with cells and bioactive substances for bioprinting applications.
This breakthrough technology is protected by
Japanese Patent No. 7696580, jointly owned by Nippi and Sunarrow Ltd. In addition, a PCT application is currently pending in the United States.
Through the distribution of this Collagen Bio-ink, Nippi aims to support medical and pharmaceutical research and drive technological progress toward clinical applications.
Demonstrated Results
The bio-ink has been successfully used to fabricate detailed human tissue-like models, including the meniscus, femur, nose, and ear. Furthermore, it was used to create a cell-laden, lattice-shaped sheet structure. The construct retained its shape after approximately one week of culture, and the cells within the structure were confirmed to be viable and properly localized.
Product Information
| Feature |
Details
|
Product Name
|
Collagen Bio-ink
|
Product Code
|
892271
|
Raw Material
|
Pepsin-solubilized collagen derived from porcine skin
|
Form
|
Syringe filled lyophilized bio-ink (0.3 g)
|
Applications
|
Construction of 3D tissue models, transplantation materials, drug delivery system carriers, and various applications in tissue engineering, regenerative medicine, and pharmaceutical research.
|
Key Features
|
High Concentration: High rigidity minimizes spreading and collapse during layer stacking, enabling vertical printing without support materials.
|
| |
High Resolution: Stable extrusion through fine nozzles (0.2 mm) enables precise fabrication of microscale and complex structures. |
| |
Excellent Operability: Room-temperature printing; neutral pH allows direct mixing with cells and bioactive substances.
|
Contact Information
For product inquiries, please contact:
Biological and Chemical Business Department
Email: protein-info@nippi-inc.co.jp